Brian Stockman

Articles

Articles

Patrone, M., Galasyn, G. S., Kerin, F., Nyitray, M. M., Parkin, D. W., Stockman, B. J. & Degano, M. (2023) A riboside hydrolase that salvages both nucleobases and nicotinamide in the auxotrophic parasite Trichomonas vaginalis, Journal of Biological Chemistry 299, 105077.

Stockman, B. J., Ventura, C. A., Deykina, V. S., Khayan Lontscharitsch, N., Saljanin, E., Gil, A., Canestrari, M. & Mahmood, M. (2023) Direct measurement of nucleoside ribohydrolase enzyme activities in Trichomonas vaginalis cells using ¹⁹F and ¹³C-edited ¹H NMR spectroscopy, Analytical Chemistry 95, 5300-5306.

Muellers, S. N., Nyitray, M. M., Reynarowych, N., Saljanin, E., Benzie, A. L., Schoenfeld, A. R., Stockman, B. J. & Allen, K. N. (2022) Structure-guided insight into specificity and mechanism of a parasitic nucleoside hydrolase, Biochemistry 61, 1853-1861.

Auletta, S., Caravan, W., Persaud, J. K., Thuilot, S. F., Brown, D. G., Parkin, D. W. & Stockman, B. J. (2019) Discovery of ligand-efficient scaffolds for the design of novel Trichomonas vaginalis uridine nucleoside ribohydrolase inhibitors using fragment screening, ACS Omega 4, 16226-16232.

Stockman, B. J., Kaur, A., Persaud, J. K., Mahmood, M., Thuilot, S. F., Emilcar, M. B., Canestrari, M., Gonzalez, J. A., Auletta, S., Sapojnikov, V., Caravan, W. & Muellers, S. N. (2019) NMR-based activity assays for determining compound inhibition, IC₅₀ values, artifactual activity, and whole cell activity of nucleoside ribohydrolases, Journal of Visualized Experiments 148, e59928.

Muellers, S. N., Gonzalez, J. A., Kaur, A., Sapojnikov, V., Benzie, A. L., Brown, D. G., Parkin, D. W. & Stockman, B. J. (2019) Ligand-efficient inhibitors of Trichomonas vaginalis adenosine/guanosine preferring nucleoside ribohydrolase,” ACS Infectious Diseases 5, 345-352.

Alam, R., Barbarovich, A. T., Caravan, W., Ismail, M., Barskaya, A., Parkin, D. W. & Stockman, B. J. (2018), Druggability of the guanosine/adenosine/cytidine nucleoside hydrolase from Trichomonas vaginalis. Chemical Biology & Drug Design 92, 1736-1742.

Beck, S., Muellers, S. N., Benzie, A. L., Parkin, D. W. & Stockman, B. J. (2015), Adenosine/guanosine preferring nucleoside ribohydrolase is a distinct, druggable antitrichomonal target. Bioorganic & Medicinal Chemistry Letters, 25, 5036-5039.

Stockman, B. J. (2015), Student-led engagement of journal article authors in the classroom using web-based videoconferencing. Journal of Chemical Education, 92, 120-123.

Stockman, B. J., Asheld, J. S., Burburan, P. J., Galesic, A., Nawlo, Z. & Sikorski, K. F. (2014), Design and characterization of a Zn2+-binding four-helix bundle protein in the biophysical chemistry laboratory. Journal of Chemical Education, 91, 451-454.

Shea, T. A., Burburan, P. J., Matubia, V. N., Ramcharan, S. S., Rosario Jr., I., Parkin, D. W. & Stockman, B. J. (2014), Identification of proton-pump inhibitor drugs that inhibit Trichomonas vaginalis uridine nucleoside ribohydrolase. Bioorganic & Medicinal Chemistry Letters, 24, 1080-1084.

Pacholec, M., Bleasdale, J. E., Chrunyk, B., Cunningham, D., Flynn, D., Garofalo, R. S., Griffith, D., Griffor, M., Loulakis, P., Pabst, B., Qiu, X., Stockman, B., Thanabal, V., Varghese, A., Ward, J., Withka, J. & Ahn, K. (2010), SRT1720, SRT2183, SRT1460, and resveratrol are not direct activators of SIRT1. Journal of Biological Chemistry, 285, 8340-8351.

Sahasrabudhe, P. V. & Stockman, B. J. (2009), Resonance assignments for stromelysin complexed with a beta-sulfonyl hydroxamate inhibitor. Biomolecular NMR Assignments, 3, 183-186.

Stockman, B. J., Kothe, M., Kohls, D., Weibley, L., Connolly, B. J., Sheils, A. L., Cao, Q., Cheng, A. C., Yang, L., Kamath, A. V., Ding, Y.-H. & Charlton, M. E. (2009), Identification of allosteric PIF-pocket ligands for PDK1 using NMR-based fragment screening and 1H-15N TROSY experiments. Chemical Biology & Drug Design, 73, 179-188.

Stockman, B. J. (2008), 2-Fluoro-ATP as a versatile tool for 19F NMR-based activity screening. Journal of the American Chemical Society, 130, 5870-5871.

Stockman, B. J., Lodovice, I. J., Fisher, D. A., McColl, A. S. & Xie, Z. (2007), An NMR-based functional assay for NAD synthetase. Journal of Biomolecular Screening, 12, 457-463.

Dalvit, C., Fagerness, P. E., Hadden, D. T. A., Sarver, R. W. & Stockman, B. J. (2003), Fluorine-NMR experiments for high-throughput screening: theoretical aspects, practical considerations and range of applicability. Journal of the American Chemical Society, 125, 7696-7703.

Dalvit, C., Hadden, D. T. A., Sarver, R. W., Ho, A. M. & Stockman, B. J. (2003), Multi-selective one dimensional proton NMR experiments for rapid screening and binding affinity measurements. Combinatorial Chemistry & High Throughput Screening, 6, 445-453.

Dalvit, C., Flocco, M., Knapp, S., Mostardini, M., Perego, R., Stockman, B. J., Veronesi, M. & Varasi, M. (2002), High-throughput NMR-based screening with competition binding experiments. Journal of the American Chemical Society, 124, 7702-7709.

Dalvit, C., Flocco, M., Veronesi, M. & Stockman, B. J. (2002), Fluorine-NMR competition binding experiments for high-throughput screening of large compound mixtures. Combinatorial Chemistry & High Throughput Screening, 5, 605-611.

Dalvit, C., Flocco, M., Stockman, B. J. & Veronesi, M. (2002), Competition binding experiments for rapidly ranking lead molecules for their binding affinity to human serum albumin. Combinatorial Chemistry & High Throughput Screening, 5, 645-650.

Chapters

Chapters

Stockman, B. J. (2016) NMR-based Activity Assays to Characterize Enzymes in the Biochemistry Laboratory and in Undergraduate Research.  In Soulsby, D., Anna, L. J., Wallner, A. S. (Eds.). NMR Spectroscopy in the Undergraduate Curriculum: Upper-Level Courses and Across the Curriculum Volume 3. (pp. 33-52). Washington, DC: American Chemical Society.

Stockman, B. J. (1996). Preparation of 2H, 13C and 15N Isotopically-Enriched Proteins for NMR Spectroscopic Investigations. In Sarkar, S. K. (Eds.). NMR Spectroscopy and its Application to Biomedical Research. (pp. 159-185). Amsterdam, Netherlands: Elsevier.

Stockman, B. J. & Markley, J. L. (1990). Stable-Isotope-Assisted Protein NMR Spectroscopy in Solution. In Bush, C. A. (Eds.). Advances in Biophysical Chemistry Volume 1. (pp. 1-46). Greenwich, CT: JAI Press.

Stockman, B. J. & Markley, J. L. (1989). Methods of Stable-Isotope-Assisted Protein NMR Spectroscopy in Solution. In Jardetzky, O. (Eds.). Protein Structure and Engineering. (pp. 152-192). New York, NY: Plenum.

Conference Presentations

Conference Presentations

Stockman, B. J., Nanda, V. & Falkowski, P. G. (2024) Prebiotic ferredoxins designed using AI deep learning algorithms, American Chemical Society Fall National Meeting, Denver, CO.

Stockman, B. J., Ventura, C. A., Deykina, V. S., Khayan Lontscharitsch, N., Saljanin, E. , Gil, A., Canestrari, M. & Mahmood, M. (2023) Translation of Nucleoside Ribohydrolase Enzyme Assays for Purified Enzymes to Direct Measurement of Enzyme Activity in Trichomonas vaginalis Cells Using ¹⁹F and ¹³C-edited ¹H NMR Spectroscopy, American Society for Biochemistry & Molecular Biology Annual Meeting, Seattle, WA.

Bonilla, J. H., Ventura, C. A. & Stockman, B. J. (2022) Computational Design of Trichomonas vaginalis Purine Nucleoside Hydrolase Inhibitors, American Chemical Society 264^th National Meeting, Chicago, IL.

Chimarios, M., Ventura, C. A. & Stockman, B. J. (2022) Parasitology Assays to Assess Trichomonas vaginalis Nucleoside Ribohydrolase Inhibitors, American Society for Biochemistry & Molecular Biology Annual Meeting, Philadelphia, PA.

Saljanin, E., Ajmal, E., Vanegas, D., VanAlstine-Parris, M. A. & Stockman, B. J. (2022) Structure-activity Relationships for Several Series of Fragment-based Inhibitors that Target Trichomonas vaginalis Nucleoside Ribohydrolase Enzymes, American Society for Biochemistry & Molecular Biology Annual Meeting, Philadelphia, PA.

Ventura, C. A., Chimarios, M., Taouil, A., Wang, H., Ojima, I., VanAlstine-Parris, M. A. & Stockman, “B. J. (2022) Concerted Use of Molecular Modeling and Parasitology to Guide Design of Novel Trichomonas vaginalis Nucleoside Ribohydrolase Inhibitors,” American Chemical Society 263^rd National Meeting, San Diego, CA.

Mahmood, M., Canestrari, M., Thuilot, S. & Stockman, B. J. (2019) “Translation of ¹H and ¹⁹F NMR-based Activity Assays to In Vitro Characterization of Nucleoside Hydrolase Activity in Cell Extracts and Whole Cells, American Society for Biochemistry & Molecular Biology Annual Meeting, Orlando, FL.

Canestrari, M., Mahmood, M., Thuilot, S. & Stockman, B. J. (2019) “Translation of ¹H and ¹⁹F NMR-based Activity Assays to In Vitro Characterization of Nucleoside Hydrolase Activity in Cell Extracts and Whole Cells, American Chemical Society 257^th National Meeting, Orlando, FL.

Kaur, A., Gonzalez, J. A., Brown, D. G., Parkin, D. W. & Stockman, B. J. (2019) “Molecular Modeling and NMR-based Counter Screens of Fragment Inhibitors of Trichomonas vaginalis Adenosine/Guanosine Nucleoside Ribohydrolase,” American Chemical Society 257^th National Meeting, Orlando, FL.

Persaud, J. K., Thuilot, S. F., Auletta, S., Caravan, W., Leonardo, A., Li, T., Dulloo, Z., Kabir, N. H., Brown, D. G., Parkin, D. W., VanAlstine-Parris, M. A. & Stockman, B. J. (2019) “Structure-activity Relationships of Fragment-based Inhibitors of Trichomonas vaginalis Uridine Nucleoside Ribohydrolase,” American Chemical Society 257^th National Meeting, Orlando, FL.

Thuilot, S. F., Persaud, J. K., Brown, D. G., Parkin, D. W. & Stockman, B. J. (2019) “NMR-based Counter Screens of Fragment Inhibitors of Trichomonas vaginalis Uridine Nucleoside Ribohydrolase Confirm Reversible, Target-specific Inhibition,” American Chemical Society 257^th National Meeting, Orlando, FL.

Auletta, S., Caravan, W., Persaud, J., Brown, D. G., Parkin, D. W. & Stockman, B. J. (2018) Identification of Ligand-efficient Inhibitors of Trichomonas vaginalis Uridine Nucleoside Ribohydrolase Using NMR-based Fragment Screening, American Chemical Society 255^th National Meeting, New Orleans, LA.

Caravan, W., Alam, R., Barbarovich, A. T., Ismail, M., Barskaya, A., Parkin, D. W. & Stockman, B. J. (2018) Ribavirin Selectively Inhibits the Guanosine/Adenosine/Cytidine Nucleoside Hydrolase from Trichomonas vaginalis, American Chemical Society 255^th National Meeting, New Orleans, LA.

Gonzalez, J. A., Kaur, A., Sapojnikov, V., Brown, D. G., Parkin, D. W. & Stockman, B. J. (2018) Emerging SAR for Several Series of Trichomonas vaginalis Adenosine/Guanosine Preferring Nucleoside Ribohydrolase Inhibitors Derived from Fragment Analog Screening, American Chemical Society 255^th National Meeting, New Orleans, LA.

Muellers, S. N., Benzie, A. L., Brown, D. G., Cowen, S., Parkin, D. W. & Stockman, B. J. (2017) Identification of Ligand-efficient Inhibitors of Trichomonas vaginalis Adenosine/guanosine Preferring Nucleoside Ribohydrolase Using NMR-based Fragment Screening, American Chemical Society 253^rd National Meeting, San Francisco, CA.

Stockman, B. J., Beck, S., Benzie, A. L., Muellers, S. N., Rosario I., Shea, T. A., Parkin, D. W. & VanAlstine-Parris, M. A. (2016) Two Distinct and Druggable Nucleoside Ribohydrolases as Targets for Trichomonas vaginalis Therapeutic Agents, 17^th Tetrahedron Symposium, Sitges, Spain.

Muellers, S. N., Beck, S., Benzie, A. L., Parkin, D. W. & Stockman, B. J. (2016) Adenosine/guanosine Preferring Nucleoside Ribohydrolase: From Flavonoids to Fragments, American Chemical Society Middle Atlantic Regional Meeting, Riverdale, NY.

Beck, S., Muellers, S. N., Benzie, A. L., Parkin, D. W. & Stockman, B. J. (2016) Adenosine/Guanosine Nucleoside Ribohydrolase is a Distinct and Druggable Antitrichomonal Target, American Chemical Society 251^st National Meeting, San Diego, CA.

Shea, T. A., VanAlstine-Parris, M. A. & Stockman, B. J. (2015). Structure-activity Relationships of Prazole Fragment Inhibitors of Trichomonas vaginalis Uridine Nucleoside Ribohydrolase Using NMR-based Activity and Binding Assays. In 249th American Chemical Society National Meeting. Denver, CO.

Shea, T. A., Burburan, P. J., Matubia, V. N., Ramcharan, S. S., Rosario I. Jr., Parkin, D. W. & Stockman, B. J. (2014). Identification of Proton-pump Inhibiting Drugs as Inhibitors of Trichomonas vaginalis Uridine Nucleoside Ribohydrolase Using an 19F NMR-based Activity Assay. In American Chemical Society 247th National Meeting. Dallas, TX.

Stockman, B. J. (2011). 19F NMR-based Activity Assays for Identifying Modulators of Function. In 35th Steenbock Symposium. Madison, WI.

Stockman, B. J., Hadden, D. T. A., Sahasrabudhe, P. V., Herberg, J. T., Hagadorn, J. C., Martin, J. P. Jr. & Rohrer, D. C. (2005). Identification of Ligand Fragments and their Binding Orientation Using WaterLOGSY and 1D Selective Inter-ligand QUIET-NOEs. In XXI International Conference on Magnetic Resonance in Biological Systems. Hyderabad, India.

Sahasrabudhe, P. V., Robarge, J. D., Hadden, D. T. A. & Stockman, B. J. (2002). To Affinity and Beyond: A Functional Genomics NMR Screening Compound Library. In XX International Conference on Magnetic Resonance in Biological Systems. Toronto, ON.

Angwin, D. T., Dalvit, C. & Stockman, B. J. (2001). Empirical Studies of WaterLOGSY and its Range of Applicability for NMR Screening. In 42nd Experimental NMR Conference. Orlando, FL.

Stockman, B. J., Farley, K. A., Angwin, D. T., Schering, C. A., Steinbrecher E. A., Shanmugasundaram, V., Lajiness, M. S., Maggiora, G. M., Bundy, G. L., Marshall, V. P., Yem, A. W., Vosters, A. F., Deibel, M. R. et al. (2000). Screening of Compound Libraries for Protein Binding Facilitated by Flow-Injection NMR Spectroscopy. In XIX International Conference on Magnetic Resonance in Biological Systems. Florence, Italy.

Stockman, B. J., Yuan, P., Waldon, D. J., Scahill, T. A., Sarver, R. W., DeZwaan, J., Belonga, K. L., Jacobsen, E. J., Treiberg, J. A., Schostarez, H. J., Mitchell, M. A., Marshall, V. P., Petzold, G. L. & Poorman, R. A. (1997). Structure, Dynamics and Calorimetry of Stromelysin/Ligand Complexes. In Keystone Symposium: Frontiers of NMR in Molecular Biology V. Taos, NM.

Stockman, B. J., Richardson, T. E. & Swenson, R. P. (1995). Structural Changes Caused by Site-Directed Mutagenesis of Tyrosine-98 in D. Vulgaris Flavodoxin Delineated by 1H and 15N NMR Spectroscopy: Implications for Redox Potential Modulation. In Keystone Symposium: Frontiers of NMR in Molecular Biology IV. Keystone, CO.

Stockman, B. J., Pollock, J. R., Scahill, T. A., Fairbanks, M. B. & Heinrikson, R. L. (1994). NMR Spectroscopy Indicates that the Mac-1 β2 Integrin α-Subunit I domain Adopts a Predominantly Folded Structure in Solution. In XVIth International Conference on Magnetic Resonance in Biological Systems. Veldhoven, Netherlands.

Stockman, B. J., Scahill, T. A., Euvrard, A., Strakalaitis, N. A., Brunner, D. P., Yem, A. W. & Deibel, M. R. Jr. (1993). Toward the Solution Structure of Interleukin-1 Receptor Antagonist Protein Determined by Heteronuclear Three-Dimensional NMR Spectroscopy. In Keystone Symposium: Frontiers of NMR in Molecular Biology III. Taos, NM.

Stockman, B. J., Scahill, T. A., Roy, M., Farley, K. A., Ulrich, E. L., Strakalaitis, N. A., Brunner, D. P., Yem, A. W. & Deibel, M. R. Jr. (1992). Secondary Structure and Topology of Interleukin-1 Receptor Antagonist Protein Determined by Heteronuclear Three-Dimensional NMR Spectroscopy. In ,” XVth International Conference on Magnetic Resonance in Biological Systems. Jerusalem, Israel.

Invited Presentations

Invited Presentations

Stockman, B. J. (2023, March) Deep Learning Approaches for Exploring the Origin of Oxidoreductases, ENIGMA Seminar Series, Rutgers University, New Brunswick, NJ.

Stockman, B. J., Brown, D. G., Hyatt, I. F. D. & VanAlstine-Parris, M. A. (2021, December) “Fragment-based Drug Discovery Targeting Trichomonas vaginalis Nucleoside Ribohydrolases,” Pacifichem 2021 Congress, Virtual.

Stockman, B. J. (2019, April) NMR-based Activity Assays to Identify and Validate Fragment Leads Against Two Trichomonas vaginalis Enzymes, North Jersey ACS NMR Topical Group, Rutgers University, Piscataway, NJ.

Stockman, B. J., VanAlstine-Parris, M. A., Parkin, D. W., Sonbuchner, T. M., Kumar, R. V. & Hyatt, I. F. D. (2019, April) Multidisciplinary Drug Discovery Research Model at an Undergraduate Institution, American Chemical Society 257^th National Meeting, Orlando, FL.

Stockman, B. J. (2019, April) Student-led Safety Inspections of Chemistry Teaching and Research Laboratories, American Chemical Society 257^th National Meeting, Orlando, FL.

Stockman, B. J. “(2019, March) Academic Honesty Awareness Week at Adelphi University,” International Center for Academic Integrity 27^th Annual Conference, New Orleans, LA.

Stockman, B. J. (2018, April) Using NMR-based Activity Assays to Identify Fragment Leads Against Two Trichomonas vaginalis Enzymes, Drug Discovery Chemistry, San Diego, CA.

Stockman, B. J. (2017, April) Biochemical NMR Spectroscopy Course for Upper Level Undergraduates that Combines Both Classroom and Laboratory Learning, American Chemical Society 253^rd National Meeting, San Francisco, CA.

Stockman, B. J., Beck, S., Bekker, S. I., Benzie, A. L., Humes, C. S., Muellers, S. N., Rosario Jr., I., Shea, T. A., Violo, V. L., Parkin, D. W. & VanAlstine-Parris, M. A. (2015, May). Nucleoside Ribohydrolases as Targets for Trichomonas vaginalis Therapeutic Agents. American Chemical Society Great Lakes/Central Regional Meeting, Grand Rapids, MI.

Stockman, B. J. (2015, March). NMR-based Activity Assays to Characterize Enzymes in the Biochemistry Laboratory and in Undergraduate Research. 249th American Chemical Society National Meeting, Denver, CO.

Stockman, B. J., Shea, T. A., Burburan, P. J., Matubia, V. N., Ramcharan, S. S., Rosario Jr., I. & Parkin, D. W. (2013, October). Identification of Trichomonas vaginalis Uridine Nucleoside Ribohydrolase Inhibitors Using an 19F NMR-based Activity Assay. American Chemical Society Northeast Regional Meeting, New Haven, CT.

Stockman, B. J. (2013, April). Crowding & Screening: Early Results From Two Undergraduate Research Projects. Hobart and William Smith College, Geneva, NY.

Stockman, B. J. (2010, October). Fragment Screening Using NMR-based Activity Assays. Connecticut College, New London, CT.

Stockman, B. J., Lodovice, I. J., Fisher, D. A., McColl, A. S. & Xie, Z. (2007, January). An NMR-based Functional Assay for NAD Synthetase. Keystone Symposium on Frontiers of NMR in Molecular Biology X, Snowbird, UT.

Stockman, B. J. (2006, September). Adding Value to NMR-Based Fragment Screens By Using Competition Binding or Functional Assays. 232nd American Chemical Society Fall National Meeting, San Francisco, CA.

Stockman, B. J. (2004, November). Competition-Based NMR Binding Assays. Eastern Analytical Symposium, Somerset, NJ.

Stockman, B. J. (2003, March). NMR Screening: Impacting Medicinal Chemistry and Biology. PITTCON 2003, Orlando, FL.

Stockman, B. J. (2000, May). Applications of Flow NMR Spectroscopy to Monitor Binding of Small Molecules to Proteins. NMR in the Drug Discovery Pipeline, London, UK.

Stockman, B. J., Scahill, T. A., Kloosterman, D. A., Jacobsen, E. J., Skaletzky, L. L., Marshall, V. P., Waszak, G. A. & Deibel, M. R. Jr. (1998, August). Solution Structure of Collagenase Complexed with a Lactam Hydroxamate Inhibitor. XVIIIth International Conference on Magnetic Resonance in Biological Systems, Tokyo, Japan.

Stockman, B. J. (1998, May). Matrix Metalloproteinase/Ligand Complexes: Structure, Dynamics, Calorimetry and Inhibitor Design. NATO Advanced Research Workshop: Applications of NMR to the Study of the Structure & Dynamics of Supramolecular Complexes, Sitges, Spain.

Stockman, B. J., Waldon, D. J., Yuan, P., Scahill, T. A., Belonga, K. L., Jacobsen, E. J., Treiberg, J. A., Schostarez, H. J., Mitchell, M. A., Liggett, W. F., Marshall, V. P., Petzold, G. L. & Poorman, R. A. (1996, August). NMR Analysis of Stromelysin/Ligand Complexes as a Tool for Structure Based Drug Design. XVIIth International Conference on Magnetic Resonance in Biological Systems, Keystone, CO.

Stockman, B. J., Scahill, T. A., Euvrard, A., Strakalaitis, N. A., Brunner, D. P., Yem, A. W. & Deibel, M. R. Jr. (1993, August). Solution Structure of Human Interleukin-1 Receptor Antagonist Protein Determined by NMR Spectroscopy. 206th American Chemical Society National Meeting, Chicago, IL.

Other Work

Other Work

Listed as inventor or co-inventor on four United States patents:
6,677,160
6,764,858
7,377,894
7,470,543

Research Interests

Research Interests

My research program contains both research and training objectives. I consider these equally important at a primarily undergraduate institution. The long-term research objective is to identify inhibitors of essential nucleoside ribohydrolase enzymes that possess efficacy against both 5-nitroimidazole-sensitive and 5-nitroimidazole-resistant strains of Trichomonas vaginalis. The training objective is achieved by immersing undergraduate students in these investigations and exposing them to cutting-edge instrumentation and research. My training and prior experience in fragment-based approaches to antibacterial and antiviral drug discovery in the pharmaceutical industry positions me to accomplish both objectives. I have established a very pragmatic undergraduate research program since moving from Pfizer to Adelphi in 2009, and have demonstrated that NMR-based screening assays used routinely in industry can also be developed and implemented for drug discovery by undergraduate students. Working together we have established the druggability of two nucleoside ribohydrolase enzymes. A fragment-based approach to identify inhibitors with novel chemical scaffolds is a logical extension of this previous work. Trichomoniasis cases resulting from strains resistant to existing drug therapies continue to increase. However, research and development of antitrichomonal agents with novel mechanisms of action is largely non-existent in the pharmaceutical industry. This creates a niche that is exquisitely matched to my expertise and interest, and a perfect opportunity to engage undergraduate students in hypothesis-driven research toward an unmet medical need. Many students that graduate from my lab go on to either graduate or professional school, so the opportunity to be part of a strong research group is a seminal experience for them. I also incorporate aspects of my research program into my biochemistry and biophysics courses which broadens the exposure to include a larger number of biology, biochemistry, and chemistry majors.

Grants

Grants

National Science Foundation, $311,035 joint with V. Nanda and P. G. Falkowski, RUI: Prebiotic Origins of Biological Energy (PrOBE). Role - Principal Investigator, 2408999, 8/1/2024-7/31/2027.

Adelphi Faculty Development Grant, $2,882, Cofactor Hopping in the Evolution of Biological Energy, awarded February 2024.

Adelphi Interdisciplinary Collaborative Grant, $2,500 joint with A. Heyl, Characterization of the Ligand Binding CHASE Domain of PpCHK4, awarded February 2019.

Adelphi Interdisciplinary Collaborative Grant, $2,750 joint with E. Villa-Cuesta, Toxicity Analysis of Treatments for Trichomoniasis Using Drosophila melanogaster, awarded February 2018.

National Institutes of Health, $310,934 joint with M. A. VanAlstine-Parris, Nucleoside Ribohydrolases as Targets for Trichomonas vaginalis Therapeutic Agents. This Project Will Evaluate Two Essential Nucleoside Ribohydrolase Enzymes as Targets for Novel Antitrichomonal Drugs. Role - Principal Investigator, R15AI128585, 6/1/2017-12/31/2021.

Adelphi Faculty Development Grant, $1,500, Nucleoside Ribohydrolase Fragment Screens to Discover Novel Antitrichomonal Chemistries, awarded February 2016.

Frederick Bettelheim Research Award, $1,200, Druggability of Adenosine/Guanosine Preferring Nucleoside Ribohydrolase, awarded April 2014.

Adelphi Faculty Development Grant, $3,450, Structure-activity Relationships of Prazole Inhibitors of Uridine Nucleoside Ribohydrolase, awarded February 2014.

Adelphi Faculty Development Grant, $2,500, Macromolecular Crowding Effects on the Structure of the Electron Transfer Protein Flavodoxin, awarded February 2012

Adelphi University Instructional Technology Grant, $2,068 joint with M. A. VanAlstine, Molecular Visualization and Presentation Software to Improve Chemistry and Biochemistry Learning Outcomes, awarded February 2011

Adelphi Faculty Development Grant, $4,250, Proof-of-concept NMR Studies on Uniformly 15N-labeled Uridine-specific N-ribohydrolase from Trichomonas vaginalis, awarded February 2010

Recent Courses

Recent Courses

Biochemistry I

Biochemistry Laboratory

Guided Research In Chemistry

Organic Chemistry I Lab

Physiological Chemistry Lab

Specialization/Interests

Specialization/Interests

Biochemistry, Biophysics and Biomolecular Structure

Honors and Accomplishments

Honors and Accomplishments

Dean’s Student Circle Outstanding Mentor Award, 2022 (Edina Saljanin)

Dean’s Student Circle Outstanding Mentor Award, 2019 (Julia Kishanie Persaud)

Dean’s Student Circle Outstanding Mentor Award, 2018 (Vital Sapojnikov)

Damon Runyon-Walter Winchell Cancer Research Fund Fellowship, 1990-1991

NIH Training Grant in Cellular and Molecular Biology, 1987-1989

Professional Activities

Professional Activities

NIH Study Section ZAI1 SAS-W (M1), 2/2022.

Editorial Board, Medicinal Chemistry Section, Molecules, 2020-

NIH Study Section ZRG1 IDM-S 83, 2018-2020

Editorial Advisory Board of Chemical Biology & Drug Design, 2007-2012

Kalamazoo Section Chair, American Chemical Society, 2001-2002

Co-General Chair, ACS Central/Great Lakes Joint Regional Meeting, 2001

Waterford Education Foundation, 2007-2010

Kalamazoo Board of Education, 1995-1999

Diplomas/Degrees

Diplomas/Degrees

M.B.A., University of New Haven (2008)

Ph.D., Biochemistry, University of Wisconsin-Madison (1989)

B.S., Chemistry and Zoology, University of Iowa (1985)